1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水,装瓶不宜过紧,用纸包好,20磅灭菌1小时。)
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 (适量)
Autoclave at 20 lbs for 1 hr.(20磅灭菌1小时)

