Peptone (蛋白胨) 10g
Glucose (葡萄糖) 2g
NaCl 5g
NaH2PO4 2.5g
Sodium citrate (柠檬酸钠) 5g
Brain infusion (脑浸汁) 800ml
Pig heart infusion (猪心浸) 200ml
Sleep Blood (羊血) 10%
pH 7.2
[Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze .
[注]猪心和猪脑浸汁法:(1)新鲜猪心除去心头和脂肪,搅碎100克加蒸馏水至1000毫升;(2)新鲜猪脑除去血管,取200克加蒸馏水至1000毫升;(3)50℃保温1小时,然后煮开15分钟;(4)冷却后用纱布过滤备用。

