Sequin is a stand-alone software tool developed by the NCBI for submitting and updating entries to the GenBank, EMBL, or DDBJ sequence databases. It is capable of handling simple submissions that contain a single short mRNA sequence, and complex submissions containing long sequences, multiple annotations, segmented sets of DNA, or phylogenetic and population studies. Getting Started with Sequin is a brief overview of the program.
Sequin 9.20 is currently available from the NCBI. Sequin runs on Macintosh, PC/Windows, and UNIX computers. Instructions for downloading and installing the program are provided. The program itself, along with its on-line help documentation, is available by anonymous FTP.
This demonstration of how to use Sequin is derived from the August 1996 issue of the NCBI News and includes a section on how to prepare your sequence data files before you begin preparing your sequence submission with Sequin.
A window containing the Sequin Help Documentation is opened when the Sequin program is launched. The contents of this scrolling window change as you move within the Sequin program, presenting you with help documentation appropriate for the section of Sequin you are presently visiting. This documentation is also available in a World Wide Web format.
A five-column, tab-delimited table of feature locations and qualifiers can be used to import annotation into an existing Sequin submission. This is the same table format that must be used to annotate features when creating a submission using tbl2asn.
Sequin can be used in one of two modes, stand-alone or network-aware. In the network-aware mode, the program can exchange data between any computer connected to the Internet and the NCBI.
The SequinMacroSend tool allows the submission of very large Sequin files directly. Those files that may be truncated during mailing with conventional maile rs, including large population sets or complete plasmids or small genomes, can be sent using this method.
To receive email notification about updates to Sequin, as well as a description of what is included in the update, follow these directions.
The tbl2asn command line program is available via ftp and is designed as an alternative to the Sequin program for generating large single submissions (complete genomes) containing a great deal of annotation. It can also be used to generate a batch submission containing thousands of individual sequences. More detailed instructions about using this function are provided. When submitting a complete bacterial genome, please review the genome guidelines.
The Sequin revision history contains a reverse chronological list of changes made to the Sequin program.
Sequin是一个独立的程序,由NCBI(美国国家生物情报中心)开发,用来向三大核酸数据库GenBank, EMBL, DDBJ 查询与提交序列数据。非常重要的一个工具软件。
相关软件和Sample下载FTP地址:ftp://ftp.ncbi.nih.gov/sequin/
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